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ptt5 based expression vectors  (Addgene inc)


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    Structured Review

    Addgene inc ptt5 based expression vectors
    Ptt5 Based Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptt5+expression+vector/pTT5-hSDC1-N20+(Plasmid+%2352355)/pm39847124-319-14-18
    Average 93 stars, based on 4 article reviews
    ptt5 based expression vectors - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Modification:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
    Article Snippet: .. All proteins used for TIRFM (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), T. thermophila CKK (residues 1635–1770), T. vaginalis CKK (residues 650–795), N. gruberi CKK (residues 612–788), P. infestans CKK (residues 1020– 1168), HsMCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells with a modified pTT5 expression vector (Addgene no. 44006 (ref. 54)) bearing Strep-GFP or Strep-SNAP tags at either the N or the C terminus of the protein. .. The cDNA sequences encoding T. thermophila, T. vaginalis and N. gruberi CKK were codon optimized for human-cell expression and synthesized as gBlocks by IDT.

    Article Title: CAMSAP-driven microtubule release from γ-TuRC and its regulation by nucleation-promoting factors
    Article Snippet: .. We used previously described SII-mCherry-CLASP2 construct . chTOG construct was a gift of S. Royle (University of Warwick, UK). chTOG-mCherry-SII was made by cloning the full length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry-Twin-Strep-tag. .. GFP-CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology, China).

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins
    Article Snippet: .. All proteins used for Total Internal Reflection Fluorescence microscopy (TIRFM) (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1 mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), Tetrahymena thermophila CKK (residues 1635–1770), Trichomonas vaginalis CKK (residues 650–795), Naegleria gruberi CKK (residues 612–788), Phytophthora infestans CKK (residues 1120–1168), human MCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells using modified pTT5 expression vector (Addgene, #44006, ) bearing strep-GFP or strep-SNAP tag at either N- or C terminus of the protein. .. The CKK cDNA sequences encoding T. thermophila , T. vaginalis , N. gruberi were codon optimized for human cell expression and synthesized as gBlock from IDT.

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, both proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE) and eluted in elution buffer (50 mM HEPES, 150 mM NaCl, 1mM MgCl 2 , 1mM EGTA, 1mM dithiothreitol (DTT), 2.5 mM d-Desthiobiotin and 0.05% Triton X-100, pH 7.4). ..

    Article Title: CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC
    Article Snippet: .. The chTOG construct was a gift from S. Royle (University of Warwick). chTOG–mCherry–SII was made by cloning the full-length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry–SII. .. GFP–CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology).

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE). ..

    Expressing:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
    Article Snippet: .. All proteins used for TIRFM (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), T. thermophila CKK (residues 1635–1770), T. vaginalis CKK (residues 650–795), N. gruberi CKK (residues 612–788), P. infestans CKK (residues 1020– 1168), HsMCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells with a modified pTT5 expression vector (Addgene no. 44006 (ref. 54)) bearing Strep-GFP or Strep-SNAP tags at either the N or the C terminus of the protein. .. The cDNA sequences encoding T. thermophila, T. vaginalis and N. gruberi CKK were codon optimized for human-cell expression and synthesized as gBlocks by IDT.

    Article Title: CAMSAP-driven microtubule release from γ-TuRC and its regulation by nucleation-promoting factors
    Article Snippet: .. We used previously described SII-mCherry-CLASP2 construct . chTOG construct was a gift of S. Royle (University of Warwick, UK). chTOG-mCherry-SII was made by cloning the full length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry-Twin-Strep-tag. .. GFP-CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology, China).

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins
    Article Snippet: .. All proteins used for Total Internal Reflection Fluorescence microscopy (TIRFM) (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1 mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), Tetrahymena thermophila CKK (residues 1635–1770), Trichomonas vaginalis CKK (residues 650–795), Naegleria gruberi CKK (residues 612–788), Phytophthora infestans CKK (residues 1120–1168), human MCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells using modified pTT5 expression vector (Addgene, #44006, ) bearing strep-GFP or strep-SNAP tag at either N- or C terminus of the protein. .. The CKK cDNA sequences encoding T. thermophila , T. vaginalis , N. gruberi were codon optimized for human cell expression and synthesized as gBlock from IDT.

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, both proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE) and eluted in elution buffer (50 mM HEPES, 150 mM NaCl, 1mM MgCl 2 , 1mM EGTA, 1mM dithiothreitol (DTT), 2.5 mM d-Desthiobiotin and 0.05% Triton X-100, pH 7.4). ..

    Article Title: CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC
    Article Snippet: .. The chTOG construct was a gift from S. Royle (University of Warwick). chTOG–mCherry–SII was made by cloning the full-length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry–SII. .. GFP–CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology).

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE). ..

    Plasmid Preparation:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins.
    Article Snippet: .. All proteins used for TIRFM (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), T. thermophila CKK (residues 1635–1770), T. vaginalis CKK (residues 650–795), N. gruberi CKK (residues 612–788), P. infestans CKK (residues 1020– 1168), HsMCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells with a modified pTT5 expression vector (Addgene no. 44006 (ref. 54)) bearing Strep-GFP or Strep-SNAP tags at either the N or the C terminus of the protein. .. The cDNA sequences encoding T. thermophila, T. vaginalis and N. gruberi CKK were codon optimized for human-cell expression and synthesized as gBlocks by IDT.

    Article Title: CAMSAP-driven microtubule release from γ-TuRC and its regulation by nucleation-promoting factors
    Article Snippet: .. We used previously described SII-mCherry-CLASP2 construct . chTOG construct was a gift of S. Royle (University of Warwick, UK). chTOG-mCherry-SII was made by cloning the full length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry-Twin-Strep-tag. .. GFP-CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology, China).

    Article Title: CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC
    Article Snippet: .. The chTOG construct was a gift from S. Royle (University of Warwick). chTOG–mCherry–SII was made by cloning the full-length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry–SII. .. GFP–CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology).

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE). ..

    Construct:

    Article Title: CAMSAP-driven microtubule release from γ-TuRC and its regulation by nucleation-promoting factors
    Article Snippet: .. We used previously described SII-mCherry-CLASP2 construct . chTOG construct was a gift of S. Royle (University of Warwick, UK). chTOG-mCherry-SII was made by cloning the full length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry-Twin-Strep-tag. .. GFP-CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology, China).

    Article Title: CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC
    Article Snippet: .. The chTOG construct was a gift from S. Royle (University of Warwick). chTOG–mCherry–SII was made by cloning the full-length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry–SII. .. GFP–CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology).

    Cloning:

    Article Title: CAMSAP-driven microtubule release from γ-TuRC and its regulation by nucleation-promoting factors
    Article Snippet: .. We used previously described SII-mCherry-CLASP2 construct . chTOG construct was a gift of S. Royle (University of Warwick, UK). chTOG-mCherry-SII was made by cloning the full length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry-Twin-Strep-tag. .. GFP-CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology, China).

    Article Title: CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC
    Article Snippet: .. The chTOG construct was a gift from S. Royle (University of Warwick). chTOG–mCherry–SII was made by cloning the full-length construct in a modified pTT5 expression vector (Addgene no. 44006) with a C-terminus mCherry–SII. .. GFP–CDK5RAP2 was a gift from Robert Z. Qi (The Hong Kong University of Science and Technology).

    Fluorescence:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins
    Article Snippet: .. All proteins used for Total Internal Reflection Fluorescence microscopy (TIRFM) (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1 mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), Tetrahymena thermophila CKK (residues 1635–1770), Trichomonas vaginalis CKK (residues 650–795), Naegleria gruberi CKK (residues 612–788), Phytophthora infestans CKK (residues 1120–1168), human MCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells using modified pTT5 expression vector (Addgene, #44006, ) bearing strep-GFP or strep-SNAP tag at either N- or C terminus of the protein. .. The CKK cDNA sequences encoding T. thermophila , T. vaginalis , N. gruberi were codon optimized for human cell expression and synthesized as gBlock from IDT.

    Microscopy:

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins
    Article Snippet: .. All proteins used for Total Internal Reflection Fluorescence microscopy (TIRFM) (human CAMSAP1 CKK (residues 1474–1613) and CAMSAP1 mini (residues 1227–1613), fly CKK (residues 1547–1689), worm CKK (residues 982–1128), Tetrahymena thermophila CKK (residues 1635–1770), Trichomonas vaginalis CKK (residues 650–795), Naegleria gruberi CKK (residues 612–788), Phytophthora infestans CKK (residues 1120–1168), human MCAK full length and human KIF5B (residues 1–560)) were expressed in HEK293T cells using modified pTT5 expression vector (Addgene, #44006, ) bearing strep-GFP or strep-SNAP tag at either N- or C terminus of the protein. .. The CKK cDNA sequences encoding T. thermophila , T. vaginalis , N. gruberi were codon optimized for human cell expression and synthesized as gBlock from IDT.

    Purification:

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, both proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE) and eluted in elution buffer (50 mM HEPES, 150 mM NaCl, 1mM MgCl 2 , 1mM EGTA, 1mM dithiothreitol (DTT), 2.5 mM d-Desthiobiotin and 0.05% Triton X-100, pH 7.4). ..

    Article Title: Structural determinants of microtubule minus end preference in CAMSAP CKK domains
    Article Snippet: .. Briefly, proteins were expressed in HEK293T cells using a modified pTT5 expression vector (Addgene no. 44006), purified using StrepTactin beads (GE). ..



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